News Details
N-hydroxy succinyl acetate coating crosslinking agent
2017-5-13 13:29:09
N-hydroxy succinyl acetate coating crosslinking agent:The NHS ester can form very stable amide bonds under the physiological pH condition and in the form of a beramine-amino acid. The protein n-amino and lysine residue of n-amino and lysine residue can form amide bonds with NHS ester.
The NHS ester, which forms a very stable amide bond, releases n-hydroxysuccinamide. The water in the NHS is hydrolyzed, and the rate of hydrolysis increases with pH.
If the concentration of protein is low, then the NHS's hydrolysis reaction will be stable. PH7.0 and 0 ℃ under the condition of bifunctional NHS ester crosslinking agent in the absence of "the hydrolysis of amino aqueous solution has a half-life of 4 yao 5 h. If the pH value of 8.6 and 4 ℃ condition, time will be shorten for 10 min.
The degree of hydrolysis of the NHS ester crosslinking agent in an aqueous solution can be detected by a 260nm OD. The n-hydroxyl amines released after hydrolysis of the NHS cause OD260nm to rise.
The hydrolyzed n-hydroxysuccinylamine has a molecular extinction coefficient of 8.2 x 103 over cm (cm/L) at 260nm under pH9 conditions. The molecular extinction coefficient in the 50mmol/L phosphate buffer solution in pH6.5 is 7.5 x 103 over cm molar.
The NHS ester can have a specific crosslinking reaction with the albumin in a non-beramine-based ion buffer solution. A common buffer system includes a phosphate buffer between 50 and 20mmol/L, a carbonate buffer, a HEPES or a boric acid salt buffer solution.
Tris is not found in the solution of the NHS ester's reaction, but it can be used for the suspension of the NHS esters. Normally, the treatment of NHS esters in neutral and alkaline conditions can be stopped by adding an excess of Tris at the end of the reaction.
Chinese name: Diethyl toluene diamine(DETDA)
Diethyltoluenediamine Uses:
The product is identical to Ethancure 100 and Lonza DETDA 80, DETDA is very effective polyurethane elastomer chain extender; also be used as polyurethane and epoxy resin curing agent, epoxy resin of an antioxidant, industrial oils and lubricants . In addition, also as intermediates in organic synthesis.Especially for the RIM (reaction injection molding), is important in the field of spray polyurea chain extender species. Also can be used for casting polyurethane elastomer (CPU) and a curing agent, epoxy curing agent, epoxy resin of antioxidants, lubricants and industrial oils other antioxidants.
Glycine can also be used to abort the reaction because it contains the beramine. The NHS ester crosslinking reaction conditions are usually 4 ℃ or room temperature, pH7-9, 30 min to 2 h reaction. 4 ℃ under the reaction conditions need to be appropriately extended time to reach the reaction efficiency at room temperature, prolonged to 4 times or so commonly.
The amount of NHS ester is two to 50 times more than the amount of protein, and the amount depends on the concentration of the protein solution. The reaction concentrations of the NHS are usually changed between 0.1 and 10 mmol/L. The concentration of a protein solution needs to be more than 10 mu of mmol/L, and at the end of 50 to 10 mu of mmol/L to avoid the hydrolysis of the crosslinking agent.
NHS ester can be divided into water soluble and water-insoluble. The water-soluble NHS ester has sulfonate on the n-hydroxysuccinamide ring, which is suitable for the crosslinking reaction that cannot tolerate organic solvents.
The sulfylated NHS ester is usually reactive in pure water, and the solubility of the DMSO is significantly reduced when there is a DMSO in the solution. Water soluble NHS ester is used primarily for molecular crosslinking reactions on the cell surface because they cannot penetrate the membrane.
It is sold in the form of sodium, which is more than 10 mmol/L. The solubility of water insoluble NHS ester varies according to the composition of the solution.
Initially, this kind of crosslinking agent is mainly dissolved in the organic solution that can be mixed with water, such as DM SO, DMF, etc. The water insoluble NHS ester does not charge so it can penetrate the cell membrane. Usually, the concentration of organic solvents is 0.5% to 10%. The NHS ester crosslinking reaction may cause the loss of protein activity, this is because the reaction of amino may reside in the active center of protein, or for the activity of protein is very important.
Copyright: Zhang Jia Gang YaRui Chemical co.,Ltd
Diethyl toluene diamine(DETDA) http://www.yaruichem.com
The NHS ester, which forms a very stable amide bond, releases n-hydroxysuccinamide. The water in the NHS is hydrolyzed, and the rate of hydrolysis increases with pH.
If the concentration of protein is low, then the NHS's hydrolysis reaction will be stable. PH7.0 and 0 ℃ under the condition of bifunctional NHS ester crosslinking agent in the absence of "the hydrolysis of amino aqueous solution has a half-life of 4 yao 5 h. If the pH value of 8.6 and 4 ℃ condition, time will be shorten for 10 min.
The degree of hydrolysis of the NHS ester crosslinking agent in an aqueous solution can be detected by a 260nm OD. The n-hydroxyl amines released after hydrolysis of the NHS cause OD260nm to rise.
The hydrolyzed n-hydroxysuccinylamine has a molecular extinction coefficient of 8.2 x 103 over cm (cm/L) at 260nm under pH9 conditions. The molecular extinction coefficient in the 50mmol/L phosphate buffer solution in pH6.5 is 7.5 x 103 over cm molar.
The NHS ester can have a specific crosslinking reaction with the albumin in a non-beramine-based ion buffer solution. A common buffer system includes a phosphate buffer between 50 and 20mmol/L, a carbonate buffer, a HEPES or a boric acid salt buffer solution.
Tris is not found in the solution of the NHS ester's reaction, but it can be used for the suspension of the NHS esters. Normally, the treatment of NHS esters in neutral and alkaline conditions can be stopped by adding an excess of Tris at the end of the reaction.
Chinese name: Diethyl toluene diamine(DETDA)
Diethyltoluenediamine Uses:
The product is identical to Ethancure 100 and Lonza DETDA 80, DETDA is very effective polyurethane elastomer chain extender; also be used as polyurethane and epoxy resin curing agent, epoxy resin of an antioxidant, industrial oils and lubricants . In addition, also as intermediates in organic synthesis.Especially for the RIM (reaction injection molding), is important in the field of spray polyurea chain extender species. Also can be used for casting polyurethane elastomer (CPU) and a curing agent, epoxy curing agent, epoxy resin of antioxidants, lubricants and industrial oils other antioxidants.
Glycine can also be used to abort the reaction because it contains the beramine. The NHS ester crosslinking reaction conditions are usually 4 ℃ or room temperature, pH7-9, 30 min to 2 h reaction. 4 ℃ under the reaction conditions need to be appropriately extended time to reach the reaction efficiency at room temperature, prolonged to 4 times or so commonly.
The amount of NHS ester is two to 50 times more than the amount of protein, and the amount depends on the concentration of the protein solution. The reaction concentrations of the NHS are usually changed between 0.1 and 10 mmol/L. The concentration of a protein solution needs to be more than 10 mu of mmol/L, and at the end of 50 to 10 mu of mmol/L to avoid the hydrolysis of the crosslinking agent.
NHS ester can be divided into water soluble and water-insoluble. The water-soluble NHS ester has sulfonate on the n-hydroxysuccinamide ring, which is suitable for the crosslinking reaction that cannot tolerate organic solvents.
The sulfylated NHS ester is usually reactive in pure water, and the solubility of the DMSO is significantly reduced when there is a DMSO in the solution. Water soluble NHS ester is used primarily for molecular crosslinking reactions on the cell surface because they cannot penetrate the membrane.
It is sold in the form of sodium, which is more than 10 mmol/L. The solubility of water insoluble NHS ester varies according to the composition of the solution.
Initially, this kind of crosslinking agent is mainly dissolved in the organic solution that can be mixed with water, such as DM SO, DMF, etc. The water insoluble NHS ester does not charge so it can penetrate the cell membrane. Usually, the concentration of organic solvents is 0.5% to 10%. The NHS ester crosslinking reaction may cause the loss of protein activity, this is because the reaction of amino may reside in the active center of protein, or for the activity of protein is very important.
Copyright: Zhang Jia Gang YaRui Chemical co.,Ltd
Diethyl toluene diamine(DETDA) http://www.yaruichem.com
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