Carbon diamide coatings crosslinking agent

Carbon diamide coatings crosslinking agent.Carbon diacamide can be mediated by the reaction of carboxyl and amino or acylhydrazine, which produces the amide bond or the hydrazone. Because the end of the protein and the side chains usually contain carboxyl and amino, the use of such crosslinking agents leads to the formation of a protein polymerase.

The covalent bond formed is similar to the peptide bond of the protein molecule, so it is very stable, and it is impossible to open the formed crosslinked bond without destroying the protein.

EDC (1 - ethyl - 3 - (3 - dimethyl ammonium propyl) - carbon 2 imide) is such a crosslinking agent in one of the most widely used, in the synthesis of haptens and enzymes used in the modification of more.

Its mechanism is thought to be the protein residues of carboxyl to react with EDC, generate lively intermediate O - acyl urea (O - acylisourea), the intermediate and nucleophilic group again, such as amino or hydroxyl, react. EDC itself can easily disassociate in water.

In addition, the high pH will increase the hydrolysis of the EDC and EDC with the activation of the carboxyl reaction. Therefore, the EDC activation carboxyl group is best performed under acidic conditions.

In practice, each time the EDC is added, the pH value will be adjusted to the range of 3. MES [2 - (morpholine) - ethane sulfonate] is a buffer applied to carbon diacamide crosslinking agent.

The phosphate buffering system reduces the efficiency of EDC, but the increase in EDC can make up for the reduction in crosslinking efficiency. Tris, glycine and acetic acid buffering systems cannot be used in EDC's crosslinking reaction system.

The NHS and sulfide NHS are often added to the EDC's response system to increase crosslinking efficiency. The EDC and the NHS use the activation of carboxyl, which is eventually available for the NHS to be activated.




Chinese name: Diethyl toluene diamine(DETDA)

Diethyltoluenediamine Raw material ：

TDA industrial, Sichuan production

Ethylene (Et) polymer grade, Shanghai production

TEA industrial imports

A reagent grade additives


This form of the isourea of the NHS is not just the same as the reactivity of the amino, but also the stability of the intermediates. O-acyl isourea itself has a half-life of only a few seconds in acidic or neutral conditions. However, the half-life can be extended up to a few hours after the activation of the NHS, which is also affected by temperature, pH and crosslinking structures.

On the structure, amide can be thought of as a compound of hydroxyl groups in the carboxylic acid molecules in the carboxylic acid group, or in the form of amino or hydrocarbon amino (-nhr or -nr2). It can also be seen as a compound of hydrogen in nitrogen atoms in ammonia or amine.

The name of the amide is based on the corresponding acyl name, and is followed by an "amine" or "certain amine", which is called "an amide" or "an amide". For example: when the amide nitrogen in the even with hydrocarbon, hydrocarbon can be written in the name of the acyl name in front of, and in the name of the hydrocarbon with "N -" "N, N -," said the hydrocarbon is linked to the nitrogen atoms.

In addition to formamide, most of the amide with RCONH2 structures is colorless solid. The fatty group replaces the RCONHR, RCONR '2, which is often liquid, and the most important of these is N, n-dimethylformamide (CH3) 2). The smaller amide can dissolve in water, as it divides

At room temperature, the other amide is a colorless crystal, except formamide is a liquid. The amide molecules contain carbonyl and amino, which can form hydrogen bonds between molecules. Due to the strong ability of the hydrogen bonding between the amide molecules, the melting point, boiling point, and even the relative quality of the carboxylic acid are high.

When the hydrogen in the nitrogen in the amide is replaced by alkyl, the association decreases, the melting point and the boiling point decrease. The fatty group n-alkyl replaces amide as a liquid. Low levels of amide are soluble in water, and the solubility decreases as the relative mass increases. Liquid amide not only dissolves organic matter, but also dissolves a lot of inorganic and is a good solvent.

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Diethyl toluene diamine(DETDA)  http://www.yaruichem.com